Background: Multiple sclerosis (MS) patients permanently confronted with serious challenges from treatment regimen. Developing a new questionnaire in MS management, through evaluation of patients’ perspectives and knowledge regarding treatment will help to identify the sources of tension, and to build a therapeutic alliance. We purposed to describe MS patients’ understanding of their treatments.Methods: About 425 completed and returned questionnaire were assessed of a total of 500 recruited MS patients. The knowledge of correct using interferon-beta (IFN-β) and attitude toward medical care were assessed using self- reported questionnaires consisted of 25 items with validity of multidisciplinary panel and pre-testing on 20 patients.Results: Knowledge about IFN-β therapy was very low; however, attitude was at a high level. Female patients, self- injection ability, higher educational level, normal functional status, delay from the start of diagnostic workup to definite diagnosis, and being younger were related to a higher level of knowledge. Attitude was associated with functional status, family history of disease and the summary of knowledge variable.Conclusion: Developing educational interventions are needed for MS patients regarding to their low levels of knowledge.